Template-directed ligation of peptides to oligonucleotides
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Template-directed ligation of peptides to oligonucleotides

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Published by National Aeronautics and Space Administration, National Technical Information Service, distributor in [Washington, DC, Springfield, Va .
Written in English

Subjects:

  • Combinatorial analysis,
  • Conjugates,
  • Pharmacology,
  • Reaction kinetics,
  • Peptides

Book details:

Edition Notes

Other titlesTemplate directed ligation of peptides to oligonucleotides.
StatementRichard K. Bruick ... [et al.].
Series[NASA contractor report] -- NASA/CR-207596., NASA contractor report -- NASA CR-207596.
ContributionsBruick, Richard K., United States. National Aeronautics and Space Administration.
The Physical Object
FormatMicroform
Pagination1 v.
ID Numbers
Open LibraryOL17582041M
OCLC/WorldCa41352574

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Rapid oligonucleotide-templated fluorogenic tetrazine ligations. Template-directed ligation of peptides to oligonucleotides. ether and 5'‐azide oligonucleotides is a particularly. The template-directed fluorogenic ligation "click" chemistry reaction was used for single nucleotide polymorphism analysis, where the target DNA catalyzes the ligation of two nonfluorescent probes. Template-Directed Oligonucleotide Strand Ligation, Covalent Intramolecular DNA Circularization and Catenation Using Click Chemistry Article in Journal of the American Chemical Society (21)   Intercalation‐mediated assembly is a powerful method for the template‐directed ligation of oligonucleotides. In their Communication on page ff., Hud and co‐workers demonstrate that proflavine, drawn in yellow, enhances the rate of oligonucleotide ligation by three orders of magnitude.

Volume 3, Issue 1 Pages (January ) Download full issue. Previous vol/issue. Book review Open archive Biotransformations in organic chemistry (2nd ed.): by Kurt Faber. Template-directed ligation of peptides to oligonucleotides. Richard K. Bruick, Philip E. Dawson, Stephen B.H. Kent, Nassim Usman, Gerald F. Joyce.   The ligation reaction of the 3’-5’ linked tetramer in the presence of a 2’-5’ decamer template or the ligation reaction of the 2’-5’ linked tetramer in the presence of a 3’-5’ decamer template took place less efficiently. The results suggest that the homo-linkage system is preferable for the template-directed synthesis of oligoRNA. We have found that nonenzymatic, template-directed ligation reactions of oligoribonucleotides display high selectivity for the formation of 3‘−5‘ rather than 2‘−5‘ phosphodiester bonds. Formation of the 3‘−5‘-linked product is favored regardless of the metal ion catalyst or the leaving group, and for several different ligation junction by: The link between non-enzymatic RNA polymerization and RNA self-replication is a key step towards the “RNA world” and still far from being solved, despite extensive research. Clay minerals, lipids and, more recently, peptides were found to catalyze the non-enzymatic synthesis of RNA oligomers. Herein, a review of the main models for the formation of the first RNA polymers is presented in Cited by: 6.

  Bruick et al. “Template-directed ligation of peptides to oligonucleotides” Chemistry and Biology, vol. 3, No. 1, Jan. , p. Tamura K, Schimmel P. “Oligonucleotide-directed peptide synthesis in a ribosome- and ribozyme-free system”.Cited by: Abstract. A protocol for the straightforward preparation of small circular oligodeoxyribonucleotides (2–28 nt) is reported. The assembly of the oligonucleotide chain (standard phosphoramidite chemistry) and cyclization by the phosphotriester method take place on a Cited by: 3. Oligonucleotide-templated reactions are useful for applying nucleic acid sensing. Various chemistries for oligonucleotide-templated reaction have been reported so far. Major scientific interests are focused on the development of signal amplification systems and signal generation systems. We introduce the recent advances of oligonucleotide-templated reaction in consideration of the above two Cited by:   The present invention is directed to the synthesis of molecules guided by connector polynucleotides (CPNs) capable of hybridizing to complementary connector polynucleotides (CCPNs.